ALDOC Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09972

Core Product Specifications and Parameters

Biological Background: ALDOC Function and Localization

• Encoded by the ALDOC gene (also known as ALDC), the protein is identified as fructose-bisphosphate aldolase C or brain-type aldolase.
• Catalyzes the reversible cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate, playing a central role in glycolysis and gluconeogenesis.
• Classified as a class I aldolase, utilizing a Schiff base intermediate for catalysis.
• Subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity or localization.
• Expressed predominantly in the brain, as indicated by its designation as brain-type aldolase, supporting high energy demands in neural tissues.
• Its enzymatic function is critical for energy metabolism, particularly in tissues with high glucose demand.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 1–170 of human ALDOC (NP_005156.1), a region that may provide strong antigenicity and specificity for the target protein.
• In western blotting, a band corresponding to the expected molecular weight of approximately 39 kDa should be observed; validate using positive-control lysates from brain or cell lines expressing ALDOC.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of antigen retrieval methods and primary antibody concentration is recommended due to possible variation in endogenous expression levels.
• The antibody cross-reacts with mouse and rat ALDOC, indicating epitope conservation; however, verifying reactivity in the specific experimental model is advisable.
• As a polyclonal antibody, minor batch-to-batch differences may occur; therefore, each new lot should be tested in a pilot study to confirm performance in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop solution for IVD raw-material procurement. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support glycolysis and energy metabolism research. From concept to clinic, we partner with you to source even the most challenging raw materials, accelerating your assay development and commercialization.