ALDH3A2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P51648

Core Product Specifications and Parameters

Biological Background: ALDH3A2 Function and Localization

• ALDH3A2 (aldehyde dehydrogenase family 3 member A2), also known as fatty aldehyde dehydrogenase (FALDH) or ALDH10, is a microsomal enzyme encoded by the ALDH3A2 gene.
• It catalyzes the oxidation of medium- and long-chain aliphatic aldehydes (C6–C24) to fatty acids, playing a key role in lipid metabolism. Related references: PMID:18035827, PMID:18182499, PMID:22633490
• ALDH3A2 is responsible for converting hexadecenal, a sphingosine 1-phosphate (S1P) degradation product, to hexadecenoic acid, linking it to sphingolipid metabolism. Related references: PMID:22633490
• The protein localizes to the endoplasmic reticulum and microsome membranes, consistent with its function in fatty aldehyde processing.
• ALDH3A2 is expressed in the liver at the protein level; its deficiency causes Sjögren-Larsson syndrome (SLS), characterized by ichthyosis and intellectual disability.
• The enzyme requires NAD as a cofactor and contains a transmembrane helix; it belongs to the aldehyde dehydrogenase superfamily.
• Alternative splicing of ALDH3A2 generates multiple isoforms, which may have distinct regulatory or functional properties.

Experimental Guidance and Technical Tips

• The immunogen (residues 250-320) is conserved in human, mouse, and rat, explaining cross-reactivity; verify sequence homology if using other species.
• For Western blot, expect a band at ~55 kDa; use liver lysate or ALDH3A2-overexpressing cells as a positive control.
• For IF/ICC, expect ER and microsome membrane staining; co-localization with ER markers (e.g., calnexin) is recommended.
• Optimize antibody dilution empirically; typical starting dilutions are 1:500–1:2000 for WB and 1:50–1:200 for IF/ICC.
• Validate the antibody in your specific experimental context, as genetic variants or post-translational modifications may affect binding.
• Store the antibody at -20°C or -80°C in aliquots after receipt to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including optimized polyclonal antibodies such as this ALDH3A2 antibody for fatty aldehyde metabolism and Sjögren-Larsson syndrome research. Our one-stop platform offers validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing, supporting your project from concept to clinic.