AIPL1 Rabbit Polyclonal Antibody (pAb) for WB, IF/ICC, ELISA - Q9NZN9

Core Product Specifications and Parameters

Biological Background: AIPL1 Function and Localization

• AIPL1 (Aryl-hydrocarbon-interacting protein-like 1), also known as AIPL2, is a member of the aryl hydrocarbon receptor-interacting protein family.
• It contains tetratricopeptide repeat (TPR) domains that facilitate protein-protein interactions, likely playing a role in protein trafficking, folding, and stabilization within the cell.
• AIPL1 is predominantly expressed in the retina, with high specificity for the developing photoreceptor layer and adult photoreceptors.
• Subcellularly, AIPL1 localizes to both the cytoplasm and the nucleus, consistent with its potential roles in protein transport and chaperone functions.
• Mutations in the AIPL1 gene are associated with Leber congenital amaurosis type 4 (LCA4), a severe early-onset retinal dystrophy leading to vision loss.
• The protein is subject to alternative splicing, and its function is linked to sensory transduction and vision per UniProt annotations.

Experimental Guidance and Technical Tips

• The immunogen spans residues 1-384 of human AIPL1, covering nearly the full-length protein, which may aid in recognizing multiple isoforms or endogenous conformations.
• For Western blotting, the predicted molecular weight is ~44 kDa; validate with appropriate positive controls such as retinal tissue or cell lines expressing AIPL1.
• In immunofluorescence/ICC, consider permeabilization protocols to detect the cytoplasmic and nuclear localization of AIPL1.
• For ELISA, the polyclonal nature enables sensitive detection; optimize coating antigen and antibody dilution in the relevant matrix.

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