AHNAK Rabbit pAb for IHC-P, IF/ICC, ELISA - Q09666

Core Product Specifications and Parameters

Biological Background: AHNAK Function and Localization

• AHNAK (Neuroblast differentiation-associated protein AHNAK), also known as Desmoyokin, is a large protein with a calculated molecular weight of approximately 629 kDa.
• It may play a role in neuronal cell differentiation, as suggested by its association with neuroblast differentiation.
• AHNAK is primarily localized to the nucleus, consistent with its potential role in transcriptional regulation or nuclear architecture.
• The protein is subject to various post-translational modifications, including acetylation, methylation, phosphorylation, ISGylation (isopeptide bond), and ubiquitination (Ubl conjugation), which may regulate its function and stability.
• Alternative splicing of AHNAK has been reported, indicating potential isoform diversity.
• The protein contains multiple repeat sequences, which may contribute to its large size and functional domains.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (amino acids 1-149) of human AHNAK. This antibody is expected to recognize epitopes within this region, but users should validate specificity in their relevant sample systems.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions and using appropriate positive and negative tissue controls. AHNAK nuclear localization should be considered when interpreting staining patterns.
• In immunofluorescence (IF/ICC), permeabilization and fixation methods may influence antigen accessibility; test conditions to confirm nuclear staining.
• For ELISA, the antibody's polyclonal nature may offer broad reactivity, but it is advisable to include proper controls to ensure signal specificity.
• Although this antibody cross-reacts with mouse and rat, confirm species compatibility by pilot experiments due to potential differences in antigen presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a one-stop platform for diagnostic manufacturers and research laboratories. Our portfolio includes validated antibodies, antigen pairs, and ancillary reagents for neuroscience and cell differentiation research, with this AHNAK antibody exemplifying our capability to supply high-quality polyclonal antibodies for rare and large protein targets. Beyond this product, CamelBio provides tailored sourcing solutions for monoclonal antibodies, bulk reagents, and custom raw-material procurement to support your entire development pipeline from concept to clinic.