AGR2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95994

Core Product Specifications and Parameters

Biological Background: AGR2 Function and Localization

• AGR2 (anterior gradient protein 2 homolog), also designated HPC8 or XAG-2 homolog, is a secreted and endoplasmic reticulum-resident protein involved in tissue homeostasis and cancer.
• It is essential for post-transcriptional synthesis and secretion of MUC2, contributing to mucosal barrier integrity in the intestine (By similarity).
• As a proto-oncogene, AGR2 promotes cell migration, differentiation, growth, and enhances cell adhesion (PubMed:23274113). Related references: PMID:23274113
• AGR2 localizes predominantly to the endoplasmic reticulum but is also secreted into the extracellular space.
• Exhibits strong expression in trachea, lung, stomach, colon, prostate, and small intestine; weaker expression in pituitary, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid, and ER-positive breast cancer cell lines.
• Contains an N-terminal signal peptide and forms a disulfide bond, consistent with its processing through the secretory pathway.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against amino acids 21–175 of mature human AGR2; for immunohistochemistry on formalin-fixed paraffin-embedded tissue, heat-induced antigen retrieval in citrate or EDTA buffer may improve staining.
• In Western blot, expect a band around 20 kDa; however, potential dimerization or glycosylation could produce additional bands—verify with appropriate controls in your sample system.
• For immunofluorescence/ICC, permeabilize cells with 0.1–0.5% Triton X-100 to detect ER-localized AGR2; paraformaldehyde fixation is recommended.
• The antibody shows cross-reactivity with mouse and rat AGR2; confirm sequence identity if precise quantification across species is needed.
• For ELISA, consider testing a range of coating antigen concentrations to optimize signal.

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CamelBio simplifies procurement for diagnostic and research organizations by offering comprehensive access to IVD raw materials, technical services, and consulting. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target sourcing, our platform supports your projects from concept to clinic. This AGR2 antibody fits into mucosal biology and oncology research, and CamelBio is ready to assist with your next development step.