AGPAT3 Rabbit Polyclonal Antibody for WB and ELISA - Q9NRZ7

Core Product Specifications and Parameters

Biological Background: AGPAT3 Function and Localization

• AGPAT3 encodes 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, also known as LPAAT-gamma, LPAAT3, or 1-AGPAT 3.
• It catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Related references: PMID:21173190
• It acts on LPA containing saturated or unsaturated fatty acids (C16:0-C20:4) at the sn-1 position, using C18:1, C20:4, or C18:2-CoA as the acyl donor. Related references: PMID:21173190
• The enzyme also possesses lysophosphatidylinositol acyltransferase (LPIAT) activity, converting lysophosphatidylinositol (LPI) to phosphatidylinositol.
• AGPAT3 localizes to the endoplasmic reticulum membrane and the nucleus envelope.
• It is widely expressed, with highest levels in testis, pancreas, and kidney, followed by spleen, lung, adipose tissue, and liver.
• The gene undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 145–310 of human AGPAT3; consider that the antibody may recognize multiple isoforms sharing this region and validate specificity in the relevant sample system.
• For Western blot, the predicted molecular weight is 43 kDa, but post‑translational modifications or alternative splicing could yield additional bands.
• In ELISA, use the recombinant immunogen as a positive control and optimize antibody dilution; cross‑reactivity with mouse allows mouse sample testing, but confirm with appropriate controls.

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CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including optimized antibodies for lipid metabolism targets such as AGPAT3. Our portfolio covers monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, supporting your assay development from concept to clinic.