AGFG1 Rabbit pAb - P52594

Core Product Specifications and Parameters

Biological Background: AGFG1 Function and Localization

• AGFG1 (Arf-GAP domain and FG repeat-containing protein 1), also designated HRB, RAB, and RIP, is a ubiquitously expressed protein containing an Arf-GAP domain and FG repeats.
• It is required for vesicle docking or fusion during acrosome biogenesis, contributing to spermatogenesis and differentiation (By similarity).
• AGFG1 may participate in mRNA transport and RNA localization, potentially influencing post-transcriptional gene regulation.
• In HIV-1 infection, AGFG1 acts as a cofactor for the viral Rev protein, facilitating the nuclear export of Rev-responsive element-containing viral RNAs, a process critical for viral replication.
• Subcellularly, AGFG1 localizes to the nucleus and cytoplasmic vesicles, consistent with its dual roles in nuclear RNA export and cytoplasmic trafficking.
• The protein features zinc-finger motifs and is subject to phosphorylation, indicating regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 1-280 amino acids of human AGFG1; consider that epitope recognition may vary among isoforms.
• For western blotting, a band near 58 kDa is anticipated; validate antibody specificity using positive and negative cell lysates.
• In ELISA applications, include appropriate controls to confirm binding and minimize background.
• Cross-reactivity with mouse AGFG1 has been demonstrated; for other species, sequence alignment is advised before use.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with this anti-AGFG1 polyclonal antibody and a wide range of IVD raw materials. As a comprehensive sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assistance in procuring rare target raw materials to support your assay development and research needs.