AFAP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N556

Core Product Specifications and Parameters

Biological Background: AFAP1 Function and Localization

• AFAP1 (Actin filament-associated protein 1), also known as AFAP-110 (110 kDa actin filament-associated protein), is a scaffold protein that links signal transduction pathways to the actin cytoskeleton.
• It can cross-link actin filaments into both network and bundle structures, modulating actin filament integrity and inducing lamellipodia formation.
• Functions as an adapter molecule that links SRC and PKC kinases to the actin cytoskeleton, potentially regulating cytoskeletal dynamics.
• Plays a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in cancer cells.
• Localizes to the cytoplasm, specifically associated with stress fibers of the cytoskeleton.
• Tissue expression varies: low in normal breast epithelial cell lines (MCF-10A) and tumorigenic breast cancer lines (MCF-7, T-47D, ZR-75-1), but highly expressed in invasive breast cancer lines (MDA-MB-231, MDA-MB-435) and overexpressed in prostate carcinoma.
• Post-translational modifications include acetylation and phosphorylation; the protein also features coiled-coil regions and repeat sequences.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 461-730, a region possibly involved in actin binding or protein interactions; consider this when designing epitope-dependent assays.
• For Western blot, lysates from invasive cancer cell lines (e.g., MDA-MB-231 for breast cancer or prostate carcinoma cells) may show stronger AFAP1 signal due to overexpression; validate in your specific sample system.
• The predicted molecular weight is ~81 kDa, but actual migration may vary; use appropriate molecular weight markers.
• The antibody has been reported to work in WB, IF/ICC, and ELISA; for immunofluorescence, permeabilization and fixation conditions should be optimized for cytoskeleton visualization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive selection of IVD raw materials, technical services, and consulting. For actin cytoskeleton and cancer research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies (like this AFAP1 antibody), bulk ancillary reagents, and tailored sourcing for rare targets. Our expertise spans every stage from concept to clinic, helping you accelerate assay development and scale-up.