ADRB1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P08588

Core Product Specifications and Parameters

Biological Background: ADRB1 Function and Localization

• ADRB1 encodes the beta-1 adrenergic receptor, a G protein-coupled receptor (GPCR) that couples primarily to G(s) proteins to activate adenylate cyclase and the cAMP-dependent pathway.
  Related references: PMID:10212248 PMID:12391161 PMID:15358775
• It binds epinephrine and norepinephrine with approximately equal affinity, mediating sympathetic nervous system responses.
  Related references: PMID:33093660
• The receptor activates Ras through binding with cAMP-dependent RAPGEF2, linking catecholamine signaling to cell growth pathways.
  Related references: PMID:12391161
• In cardiac muscle, ADRB1 stimulates cardiomyocyte contraction and is essential for normal heart function.
  Related references: PMID:11052857
• It also transmits proapoptotic signals in cardiomyocytes, indicating a role in cardiac pathology.
• ADRB1 contributes to sleep/wake cycle regulation.
  Related references: PMID:11854867 PMID:31473062
• The receptor localizes to the cell membrane and undergoes phosphorylation, palmitoylation, and N-linked glycosylation, which modulate its function and desensitization.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the intracellular region (aa 150–250) of human ADRB1; thus, the antibody may recognize both unmodified and post-translationally modified forms in denaturing Western blot.
• For Western blot, a primary band near 51 kDa is expected; consider that additional bands could arise from receptor dimerization or limited proteolysis.
• In immunofluorescence (IF-P), anticipate membrane-associated staining; punctate patterns may reflect receptor internalization or clustering.
• Reported cross-reactivity with mouse and rat enables use in rodent cardiac models; nevertheless, validation in the specific experimental system is recommended.
• For ELISA, the antibody may be suitable as a capture or detection reagent; optimal titer and working dilution should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers, offering a streamlined supply chain for IVD raw materials. For GPCR targets such as ADRB1, we can provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support immunoassay development. With expertise in rare-target sourcing, CamelBio helps accelerate your project from concept to clinic.