ADORA2A Rabbit pAb - P29274

Core Product Specifications and Parameters

Biological Background: Adenosine Receptor A2a (ADORA2A) Function and Localization

• ADORA2A encodes the adenosine receptor A2a, a member of the G protein-coupled receptor (GPCR) family.
• It functions as a receptor for adenosine, and its activity is mediated by G proteins, primarily Gs, which activate adenylyl cyclase and increase cAMP levels.
• The receptor is predicted to contain seven transmembrane domains, characteristic of GPCRs, and possesses a disulfide bond and glycosylation sites.
• Subcellularly, ADORA2A is located at the cell membrane, consistent with its role in signal transduction from extracellular adenosine.
• The protein is subject to ubiquitination (Ubl conjugation), suggesting regulation by proteasomal degradation.
• ADORA2A is widely studied in the context of neurodegenerative diseases, cardiovascular function, and inflammation, due to its modulatory roles.
• Alternative names include A2aR, RDC8, and ADORA2.
• The recombinant immunogen sequence corresponds to amino acids 300–400 of the human protein (NP_000666.2), a region likely within the intracellular C-terminal domain.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the intracellular C-terminus (aa300–400), which may yield an antibody suitable for detecting total ADORA2A protein levels in denatured samples (WB).
• For WB, a band at approximately 45 kDa is expected, though post-translational modifications may cause shifts.
• Given cross-reactivity with mouse and rat, it is suitable for studies across these species; however, sequence alignment of the immunogen region is recommended to ensure homology.
• For IF/ICC, fixation and permeabilization conditions should be optimized, as the target is a transmembrane protein; testing both methanol and formaldehyde fixation is advised.
• Always include appropriate controls (e.g., knockout cell lines or peptide blocking) to confirm specificity in your experimental system.

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