ADAMTS13 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q76LX8

Core Product Specifications and Parameters

Biological Background: ADAMTS13 Function and Localization

• ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) is also known as von Willebrand factor-cleaving protease (vWF-CP). It is a secreted glycoprotein with multiple domains including a metalloprotease domain and thrombospondin type-1 repeats.
• The primary function of ADAMTS13 is to cleave large von Willebrand factor (vWF) multimers in plasma into smaller, less thrombogenic forms, thereby regulating vWF-mediated platelet thrombus formation.
• ADAMTS13 is primarily expressed in the liver and secreted into plasma, where it maintains hemostatic balance.
• The protein is synthesized as a zymogen and undergoes proteolytic activation. It contains disulfide bonds, binds zinc and calcium, and is a member of the metalloprotease family.
• Keywords associated with ADAMTS13 include: Blood coagulation, Hemostasis, Hydrolase, Metalloprotease, Secreted, Glycoprotein, and Disease variant, highlighting its role in thrombotic disorders such as thrombotic thrombocytopenic purpura.

Experimental Guidance and Technical Tips

• For Western blotting, use standard protein extraction and loading. The predicted molecular weight is 154 kDa; ensure sufficient electrophoresis time and transfer conditions for high-molecular-weight proteins. Depending on sample processing, truncated or processed forms may appear.
• For immunofluorescence on fixed cells or tissues, permeabilization may be required for intracellular detection, though ADAMTS13 is secreted. Consider analyzing liver tissue sections or transfected cells.
• ELISA performance has been validated; consider optimizing antibody concentration and blocking conditions for specific sample types.
• Since the immunogen is a peptide from the N-terminal region (aa 50-150), the antibody may recognize epitopes specific to the propeptide or catalytic domain. It might detect both pro-form and mature enzyme.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic manufacturers, laboratories, and research institutes. If you are developing assays targeting ADAMTS13 for thrombotic or hemostatic disorders, our team can support you with customized raw-material solutions from concept to clinic.