ADAM9 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13443

Core Product Specifications and Parameters

Biological Background: ADAM9 Function and Localization

• ADAM9 is a disintegrin and metalloproteinase domain-containing protein 9, also known as meltrin-gamma, MCMP, MDC9, or CORD9. It is a member of the ADAM family and plays key roles in cell adhesion and proteolysis.
• Functions as a metalloprotease that cleaves and releases molecules important for tumorigenesis and angiogenesis, including TEK, KDR, EPHB4, CD40, VCAM1, and CDH5.
• May act as an alpha-secretase for amyloid precursor protein (APP), contributing to non-amyloidogenic processing.
• Mediates cell-cell and cell-matrix interactions and may regulate cell motility through interactions with integrins.
• Subcellular localization includes the cell membrane and also secreted forms.
• Widely expressed in various tissues, with isoform 2 highly expressed in liver and heart. Also expressed in chondrocytes.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; contains a zinc-binding motif for its protease activity.
• Associated with cone-rod dystrophy through alternative splicing and mutations.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 29-210) of human ADAM9, which includes the prodomain and part of the metalloprotease domain. For Western blotting, consider using reducing conditions and validate the full-length band at ~91 kDa as well as possible processed forms.
• For immunofluorescence (IF/ICC), expect membrane localization; appropriate fixation and permeabilization may be needed for optimal detection.
• ELISA applications can be optimized by coating with the recombinant immunogen or native samples. Ensure to validate the antibody in your specific experimental system, as performance may vary.
• This antibody is reported to cross-react with human, mouse, rat, and monkey; validate in your target species and tissue prior to large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-ADAM9 antibody, to support tumor biology and angiogenesis research. From antibody pairs to recombinant proteins and ancillary reagents, we provide bulk and custom sourcing solutions for diagnostic manufacturers and research institutes. Contact us to discuss your project and simplify your supply chain from concept to clinic.