ADAM28 Rabbit pAb for WB and ELISA - Q9UKQ2

Core Product Specifications and Parameters

Biological Background: ADAM28 Function and Localization

• ADAM28 (Disintegrin and metalloproteinase domain-containing protein 28) is a member of the ADAM family of transmembrane/secreted multi-domain metalloproteases.
• Alternative splicing generates multiple isoforms; isoform 2 is preferentially expressed in the spleen.
• ADAM28 functions in lymphocyte emigration by mediating adhesive and proteolytic events, and may shed ectodomains of surface targets like FASL and CD40L.
• It is also implicated in sperm maturation.
• Subcellularly, ADAM28 localizes to the cell membrane and can be secreted.
• The protein contains EGF-like and disintegrin domains, requires zinc for catalytic activity, and undergoes zymogen activation by cleavage on basic residues.
• ADAM28 expression is enriched in secondary lymphoid tissues such as lymph node, spleen, small intestine, stomach, colon, appendix, and trachea, with lymphocyte populations responsible in these tissues.

Experimental Guidance and Technical Tips

• For Western blotting, optimize primary antibody dilution (e.g., 1:500–1:2000) and validate using lysates from lymphoid tissues (e.g., spleen, lymph node) or cell lines expressing ADAM28.
• In ELISA, the recombinant protein immunogen can serve as a positive control to assess antibody binding and assay sensitivity.
• The antibody reacts with human ADAM28 and is predicted to recognize mouse and rat orthologs due to sequence homology; confirm cross-reactivity in your model system.
• Multiple isoforms exist; the canonical full-length protein is 87 kDa, but additional bands may appear corresponding to alternative splicing or proteolytic processing.
• Consider the functional implications of ADAM28's shedding activity when designing experiments; include appropriate protease inhibitors in lysis buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for immunoassay development. This ADAM28 antibody supports research into lymphocyte function and reproductive biology, and we can assist with bulk orders or custom sourcing of rare targets. From concept to clinic, CamelBio streamlines your procurement of high-quality raw materials.