ADAM19 Rabbit Polyclonal Antibody for WB, ELISA - Q9H013

Core Product Specifications and Parameters

Biological Background: ADAM19 Function and Localization

• ADAM19 (Disintegrin and metalloproteinase domain-containing protein 19) is also known as Meltrin-beta and MADDAM (Metalloprotease and disintegrin dendritic antigen marker).
• It belongs to the ADAM family of transmembrane metalloproteases, characterized by disintegrin and metalloproteinase domains, along with EGF-like, transmembrane, and cytoplasmic SH3-binding motifs.
• ADAM19 participates in the proteolytic processing of beta-type neuregulin isoforms, which are crucial for neurogenesis and synaptogenesis, potentially regulating glial cell functions.
• It also cleaves alpha-2 macroglobulin, suggesting broader substrate recognition.
• Functional studies indicate a possible role in osteoblast differentiation and bone homeostasis.
• Subcellularly, ADAM19 is a membrane protein with a single-pass transmembrane helix.
• The protein is widely expressed across normal organ tissues and is also detected in various cancer cell lines.
• Post-translational modifications include glycosylation, and the protein contains multiple disulfide bonds; it is synthesized as a zymogen requiring activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 203-460) of human ADAM19, which includes part of the disintegrin and cysteine-rich domains. This design may facilitate recognition of native and processed forms.
• For Western blot, a band around 105 kDa is expected for the full-length pro-form, but consider that ADAM19 undergoes proteolytic processing, so additional lower molecular weight bands may appear.
• ELISA applications can be optimized using the recombinant immunogen as coating antigen; consider testing different antibody dilutions.
• Cross-reactivity is reported for human, mouse, and rat, but sequences in the immunogen region are not identical across species; validation is recommended for each assay system.
• As a polyclonal antibody, batch-to-batch variability can occur; it is advisable to include appropriate controls and evaluate lot-specific performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a global one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with seamless access to high-quality antibodies and ancillary reagents. For ADAM19 research—spanning neurobiology, oncology, and bone biology—we offer validated polyclonal and monoclonal options, bulk supply, and custom sourcing support to accelerate your assay development from concept to clinic.