ACTR1B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42025

Core Product Specifications and Parameters

Biological Background: ACTR1B Function and Localization

• ACTR1B encodes beta-centractin, also known as actin-related protein 1B (ARP1B), a member of the actin superfamily that shares structural homology with cytoskeletal actins.
• Beta-centractin is a core subunit of the dynactin complex, a multi-subunit assembly that links dynein motors to cargo vesicles and organelles, facilitating minus-end-directed transport along microtubules.
• The protein localizes predominantly to the centrosome, a major microtubule-organizing center, where it contributes to microtubule anchoring and organization.
• Subcellular distribution is not limited to centrosomes; beta-centractin is also found throughout the cytoplasm and associated with the cytoskeleton.
• As noted in UniProt keywords, the protein is subject to post-translational modifications including acetylation and nitration, and contains an ATP-binding site, suggesting regulated activity.
• With a calculated molecular weight of approximately 42 kDa, beta-centractin is conserved across species and plays a fundamental role in intracellular trafficking and spindle orientation during mitosis.
• The ACTR1B gene is referred to under aliases such as CTRN2, PC3, and ARP1B.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein covering the N-terminal 105 amino acids of human ACTR1B. This region is likely conserved, consistent with the observed cross-reactivity with mouse and rat.
• For Western blot (WB), a specific band around 42 kDa is expected; consider optimizing blocking and antibody dilution using a relevant positive control lysate.
• Immunohistochemistry on paraffin-embedded sections (IHC-P) may reveal centrosomal and cytoplasmic staining; validate with known centrosome markers in your tissue of interest.
• In ELISA, the antibody can be employed as a capture or detection reagent; pair with an appropriate secondary antibody and validate linearity using recombinant ACTR1B protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ACTR1B polyclonal antibody supports research into microtubule-based transport and centrosome biology. We supply a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target raw materials to accelerate your assay development.