Activator protein 2 (AP-2/TFAP2A) Rabbit pAb - P05549

Core Product Specifications and Parameters

Biological Background: AP-2/TFAP2A Function and Localization

• Sequence-specific transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3' to regulate gene expression.
• Activates genes essential for embryonic development, including eye, face, body wall, limb, and neural tube formation, and is uniquely required for early lens vesicle morphogenesis.
• Functions as a transcriptional repressor for genes like MCAM/MUC18, C/EBP alpha, and MYC, and negatively regulates Wnt canonical signaling.
  Related references: PMID:33000225
• Cooperates with the CITED2 coactivator to stimulate PITX2 P1 promoter transcription and associates with the PITX2 P1 promoter region.
• Localizes to the nucleus and undergoes post-translational modifications including phosphorylation and ubiquitination, as indicated by keywords Phosphoprotein and Ubl conjugation.
• Belongs to the AP-2 transcription factor family; multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider loading 20-30 µg of total protein lysate and testing antibody dilutions ranging from 1:500 to 1:2000 to optimize signal detection at the predicted molecular weight of ~48 kDa.
• The immunogen (residues 100-200) is conserved in human and rat; cross-reactivity has been confirmed, but sequence alignment is advised before testing other species.
• Validate the antibody in your specific ELISA setup by titrating to determine optimal coating or detection concentrations.
• Include appropriate positive and negative controls, such as nuclear extracts from HeLa cells, to confirm specificity.

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CamelBio is your trusted one-stop partner for IVD raw materials, offering validated antibody pairs, optimized mono/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For transcription factor research and developmental biology, we supply high-quality antibodies like anti-TFAP2A to support your assay development from concept to clinic. Contact our team to explore custom sourcing solutions and accelerate your diagnostic projects.