ACTC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P68032

Core Product Specifications and Parameters

Biological Background: ACTC1 Function and Localization

• Alpha-cardiac actin encoded by ACTC1 is a member of the actin family, which are highly conserved proteins involved in various types of cell motility.
• It is a key component of the cytoskeleton in cardiac muscle cells, contributing to muscle contraction and structural integrity.
• The protein localizes to the cytoplasm and cytoskeleton, where it polymerizes to form microfilaments.
• Post-translational modifications such as acetylation, methylation, and oxidation may regulate its function and interactions.
• Mutations in ACTC1 are associated with diseases including dilated cardiomyopathy (CMD1R), hypertrophic cardiomyopathy (CMH11), atrial septal defect (ASD5), and left ventricular noncompaction (LVNC4).
• As a ubiquitous cytoskeletal protein, ACTC1 also serves as a reference proteome for proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of ACTC1, which may confer specificity to the cardiac isoform.
• For Western blot, the expected band is ~42 kDa; consider using heart tissue lysates as a positive control.
• For IHC-P, optimize antigen retrieval conditions to achieve specific staining.
• For ELISA, validate the optimal coating concentration using recombinant ACTC1 protein.
• Cross-reactivity with mouse and rat suggests utility in rodent models; confirm sequence homology of the epitope across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable, one-stop access to high-quality IVD raw materials. Our ACTC1 rabbit polyclonal antibody supports cardiac muscle research and assay development. In addition to validated antibodies, we offer customized polyclonal and monoclonal antibody development, bulk reagent supply, and technical consulting to streamline your workflow from concept to clinic.