ACSL5 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9ULC5

Core Product Specifications and Parameters

Biological Background: ACSL5 Function and Localization

• ACSL5, also known as ACS5 or FACL5, encodes long-chain-fatty-acid--CoA ligase 5, which catalyzes the ATP-dependent activation of long-chain fatty acids into acyl-CoA thioesters for both cellular lipid synthesis and mitochondrial β-oxidation. Related references: PMID:17681178, PMID:22633490, PMID:24269233
• It exhibits a broad substrate range with a preference for C16-C18 unsaturated fatty acids (By similarity).
• ACSL5 may direct exogenous fatty acids into triacylglycerol storage and may also stimulate fatty acid oxidation (By similarity).
• At the villus tip of the small intestine, ACSL5 sensitizes epithelial cells to TRAIL-mediated apoptosis, suggesting a role in epithelial turnover.
• It has been implicated in the survival of glioma cells, indicating a potential function in cancer biology.
• The protein localizes to the mitochondrial outer membrane, endoplasmic reticulum membrane, and cell membrane, consistent with its roles in lipid metabolism and signaling.
• ACSL5 undergoes alternative splicing, acetylation, and phosphorylation, which may regulate its activity and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 500–683), which may allow detection of multiple ACSL5 splice variants if the epitope is conserved. Verify in relevant samples.
• For Western blotting, expect a band near 76 kDa. Include appropriate positive and negative controls to confirm specificity.
• In immunofluorescence experiments, optimize permeabilization conditions to account for the protein's membrane‐associated localization.
• For IHC-P, antigen retrieval and detection protocols should be individually optimized. Validate staining patterns in tissue sections with known ACSL5 expression.
• This monoclonal antibody can be used in ELISA; consider pairing with a suitable detection antibody for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers and laboratories with high‐quality reagents like this ACSL5 antibody. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or custom sourcing for rare targets, CamelBio supports your workflow from concept to clinic. Our expertise in lipid metabolism and oncology‐related markers ensures you receive reliable tools for your research and development needs.