ACOT9 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y305

Core Product Specifications and Parameters

Biological Background: ACOT9 Function and Localization

• ACOT9 (Acyl-CoA thioesterase 9) is a mitochondrial enzyme that hydrolyzes acyl-CoA esters to free fatty acids and CoA, modulating their cellular pools.
• It participates in the regulation of both mitochondrial lipid and amino acid metabolism, linking these two metabolic pathways.
• The protein localizes to the mitochondrion, with reports indicating its presence in the mitochondrial matrix and association with the inner membrane.
• ACOT9 is also referred to as ACATE2, CGI-16, MTACT48, and MT-ACT48, reflecting its identification in various screens.
• UniProt annotations highlight its involvement in fatty acid metabolism and lipid metabolism, and classify it as a serine esterase and hydrolase.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• The canonical ACOT9 protein has a predicted molecular weight of approximately 50 kDa (calculated 49902 Da).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 22–250 of human ACOT9, which covers the N-terminal region; consider this when assessing isoform detection.
• For Western blotting, a band around 50 kDa is expected; validate the signal using mitochondrial-enriched lysates or recombinant protein controls.
• In immunofluorescence/immunocytochemistry, anticipate a mitochondrial staining pattern; co-staining with a mitochondrial marker is recommended to confirm localization.
• The antibody has been reported to cross-react with mouse and rat ACOT9; verify sequence homology and test in your sample species to ensure specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; consider including appropriate positive and negative controls in each experiment.
• For ELISA applications, the antibody may be used for direct or capture detection; titration and optimization in your assay system are essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive support for diagnostic manufacturers and research laboratories, providing high-quality IVD raw materials such as optimized antibodies, antibody pairs, and ancillary reagents. With the ACOT9 Rabbit Polyclonal Antibody, we facilitate studies in mitochondrial metabolism and related disorders, leveraging our supply chain to deliver consistent, bulk quantities. Our team also assists with custom sourcing for rare targets, ensuring you have the critical tools for your development pipeline from concept to clinic.