Acetyl-p53 (K370) Rabbit Monoclonal Antibody (P04637) for Western Blot and ELISA

Core Product Specifications and Parameters

Biological Background: p53 (TP53) Function and Localization

p53 (Cellular tumor antigen p53, TP53) is a well-characterized tumor suppressor protein that functions as a multifunctional transcription factor. It induces cell cycle arrest, DNA repair, or apoptosis upon binding to its target DNA sequences, thereby maintaining genomic stability and preventing cancer development (PubMed:11025664, 12524540, 12810724, 15340061, 17317671, 24051492).

Related references: PMID:11025664, PMID:12524540, PMID:12810724, PMID:15340061, PMID:17317671, PMID:24051492

Subcellular localization studies show that p53 is predominantly nuclear under normal conditions but can translocate to the cytoplasm, mitochondria, PML bodies, and endoplasmic reticulum in response to various cellular stresses. This dynamic compartmentalization is critical for its diverse functions, including transcription regulation and induction of apoptosis (PubMed:15340061, 22726440, 11025664, 12810724, 17170702, 25168243, 27323408, 28842590).

Related references: PMID:15340061, PMID:22726440, PMID:11025664, PMID:12810724, PMID:17170702, PMID:25168243, PMID:27323408, PMID:28842590

p53 is ubiquitously expressed in most tissues, with multiple isoforms exhibiting distinct expression patterns. The main isoform 1 is broadly expressed, while other isoforms show tissue-specific presence or absence, adding a layer of regulatory complexity (PubMed:16131611).

Related references: PMID:16131611

The activity of p53 is tightly controlled by extensive post-translational modifications (PTMs), including phosphorylation, acetylation, methylation, and ubiquitination. Lysine 370 (K370) in the C-terminal regulatory domain is a hotspot for such modifications, undergoing methylation and dimethylation by enzymes like SMYD2 and others, which modulate p53 stability and transcriptional activity. This antibody was generated using a synthetic acetylated peptide around K370 of human p53 (P04637), specifically recognizing the acetylated form at this residue. Acetylation at K370 is associated with enhanced DNA binding and activation of p53 target genes, linking it to tumor suppression.

Related references: PMID:17108971, PMID:22864287 (For methylation/dimethylation at K370)

Experimental Guidance and Technical Tips

• The antibody is raised in rabbit and is monoclonal, ensuring high specificity and consistency across batches.
• For Western blotting, consider enriching nuclear protein extracts, as p53 is predominantly nuclear; however, depending on cellular stress conditions, p53 may also be present in cytoplasmic or mitochondrial fractions.
• Validate antibody performance in positive control samples known to express acetylated p53, such as cells treated with deacetylase inhibitors (e.g., trichostatin A or nicotinamide) or DNA‑damaging agents (e.g., etoposide, doxorubicin).
• When using ELISA, coat the plate with the immunogen peptide (acetylated) and a non‑acetylated control peptide to confirm specificity.
• Cross‑reactivity is confirmed for human and mouse, but consider sequence alignment if testing other species.
• Optimize working dilutions empirically; recommended starting dilutions for WB: 1:500‑1:2000, for ELISA: 1:1000‑1:5000 (to be titrated by the end‑user).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering a one-stop solution from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized rare target materials to support your diagnostic and research needs. This acetyl-p53 (K370) rabbit monoclonal antibody (P04637) is a high-quality tool for apoptosis and cancer research, exemplifying our commitment to delivering critical reagents for understanding tumor suppression mechanisms. Partner with CamelBio to streamline your procurement and accelerate your projects.