Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (P62805) for WB, ChIP, CUT&Tag

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, essential for DNA compaction into chromatin and regulation of transcription, replication, DNA repair, and chromosomal stability. DNA accessibility is governed by a complex set of post-translational modifications known as the "histone code."

Acetylation of histone H4 at lysine 5 (K5; corresponding to Lys-6 in UniProt P62805) is a pivotal modification associated with active chromatin states. This mark is deposited by histone acetyltransferases (HATs) and removed by deacetylases (HDACs), dynamically influencing gene expression. Acetylated H4K5 is enriched at promoter regions of actively transcribed genes and contributes to chromatin decompaction and transcriptional activation. Related references: PMID:2474456, PMID:7664735, PMID:19608861

Histone H4 is encoded by multiple gene copies (H4C1–H4C16) located in histone clusters on chromosomes 1 and 6. The protein is highly conserved across species, which explains the predicted wide cross-reactivity of this antibody. H4 is subject to extensive combinatorial modifications; K5 can also undergo other acylations such as crotonylation, butyrylation, and 2-hydroxyisobutyrylation, highlighting the complexity of the histone code.

Subcellularly, histone H4 is primarily nuclear and associates with chromosomes. During spermatogenesis, acetylated H4 localizes to the nucleus in step 11 spermatids. The N-terminal tail modifications, including K5 acetylation, play pivotal roles in chromatin remodeling and the recruitment of effector proteins. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic acetylated peptide spanning lysine 5 of human histone H4. It is expected to specifically recognize H4 acetylated at K5 (Lys-6 in UniProt P62805) and may have minimal cross-reactivity to unmodified H4 or other acetylated lysines.
• For Western blot (WB), consider using histone-enriched or acid-extracted protein preparations to enhance signal. The predicted molecular weight of histone H4 is 11 kDa.
• In immunoprecipitation (IP) and chromatin-based applications (ChIP, ChIP-seq, CUT&Tag), titrate the antibody and optimize cross-linking/fixation conditions. The wide predicted species cross-reactivity (human, mouse, rat, and others) suggests utility across mammalian models, but validation in the specific sample system is recommended.
• For immunohistochemistry (IHC-P) and ELISA, ensure appropriate controls (e.g., non-acetylated peptide competition) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. For epigenetic targets like acetyl-histone H4 (P62805), we supply high-quality antibodies for applications including WB, ChIP, and CUT&Tag, alongside custom sourcing solutions for rare or emerging modification-specific reagents. Contact us for one-stop support from concept to clinic.