Acetyl-Histone H4 K91 Rabbit Polyclonal Antibody (P62805) – WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is one of the core histones that form the nucleosome, the fundamental unit of chromatin. Together with H2A, H2B, and H3, it wraps DNA around the histone octamer, compacting the genome and regulating access for transcription, replication, and repair. Histone H4 plays a central role in the dynamic regulation of chromatin structure through a vast array of post-translational modifications (PTMs), collectively known as the "histone code." These modifications include acetylation, methylation, phosphorylation, ubiquitylation, and many others, which occur predominantly on the N-terminal tail but also on the globular domain.

Acetylation of specific lysine residues on histone H4 is associated with transcriptional activation and open chromatin structure. Lysine 91 (K91), located within the globular domain of H4, is one such residue that can be acetylated. Acetylation at K91 (corresponding to K92 in UniProt isoform P62805) has been shown to regulate chromatin dynamics and may influence DNA repair processes. Related references: PMID:19818714

The highly conserved nature of histone H4 across eukaryotes ensures that acetylation-specific antibodies like this anti-acetyl-H4-K91 exhibit broad cross-reactivity, making them valuable tools for epigenetic studies in multiple species. H4 is predominantly localized in the nucleus, associated with chromosomes, and is present throughout the cell cycle, with specific acetylation patterns changing during differentiation and development. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• For Western blot (WB), use histone-enriched nuclear extracts or acid-extracted histones to improve detection. Consider including a negative control such as a non-acetylated peptide or a deacetylase inhibitor (e.g., TSA) treatment to confirm specificity for the acetylated K91 form. The predicted molecular weight of histone H4 is approximately 11 kDa, but migration may vary slightly depending on PTMs.
• In immunofluorescence / immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions (e.g., 4% PFA or methanol fixation, followed by 0.1% Triton X-100) to preserve the epitope. Co-staining with DNA dyes (e.g., DAPI) can confirm nuclear localization.
• For ELISA applications, use synthetic acetylated K91 peptide versus unmodified peptide as coating antigens to assess antibody specificity and titer. This antibody is predicted to cross-react with mouse, rat, and other species due to the high sequence homology of histone H4, but verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinical application. For your histone modification and epigenetic research needs, we provide this high-quality rabbit polyclonal antibody against acetyl-histone H4-K91, along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio streamline your supply chain and accelerate your diagnostic and research projects.