Acetyl-Histone H4 (K8) Rabbit Polyclonal Antibody (P62805) - WB, IHC-P, IF/ICC, ELISA, ChIP, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, thereby restricting DNA accessibility to cellular machineries that require DNA as a template. Through these interactions, histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.

DNA accessibility is governed by a complex set of post-translational modifications (PTMs) on histones, collectively referred to as the "histone code," as well as by nucleosome remodeling. Histone H4 is extensively modified, particularly on its N-terminal tail, with marks including acetylation, methylation, phosphorylation, citrullination, and ubiquitination.

Acetylation at lysine 8 (K8) of histone H4—corresponding to position 9 in the UniProt sequence (P62805)—is a well-characterized modification associated with open chromatin and transcriptional activation. This modification is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs). Related references: PMID:2474456, PMID:7664735, PMID:19608861.

Histone H4 is primarily localized in the nucleus and on chromosomes. In certain cell types, such as spermatids, acetylated H4 is observed to be concentrated in the nucleus during specific developmental stages.

Histone H4 is encoded by multiple gene copies, including HIST1H4A through HIST1H4L, HIST2H4A/B, and HIST4H4, which produce identical protein sequences. This high conservation across species underpins its wide predicted cross-reactivity.

Experimental Guidance and Technical Tips

• For ChIP and ChIP-seq, consider optimizing the cross-linking and chromatin shearing conditions to preserve the labile acetyl modification. Use of histone deacetylase inhibitors (e.g., sodium butyrate, TSA) during cell harvesting may enhance detection.
• In Western blotting (WB), it is recommended to include both acetyl-histone H4 peptide and non-acetylated peptide as blocking controls to confirm antibody specificity.
• Because histone H4 is highly conserved, this antibody is expected to cross-react with a wide range of species; however, validation in the relevant sample system is advisable.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols should be carefully evaluated, as the acetyl epitope may be sensitive to harsh treatments.
• When using this antibody, lot-to-lot consistency should be monitored, especially in quantitative assays, as is standard for polyclonal reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a one-stop portfolio of validated IVD raw materials, including this acetyl-specific histone H4 antibody for epigenetics and chromatin research. Our supply capabilities encompass optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, all designed to support your assay development from concept to clinic. Let CamelBio be your reliable partner in streamlining procurement for next-generation diagnostic products.