Acetyl-Histone H4-K5 Rabbit mAb (P62805) for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, the fundamental unit of chromatin. Nucleosomes wrap DNA, compacting it and regulating accessibility for transcription, DNA repair, replication, and chromosomal stability. The modulation of DNA accessibility is orchestrated through a complex array of post-translational modifications, known as the histone code.
  Related references: UniProt P62805.

• Histone H4 localizes predominantly to the nucleus and is associated with chromosomes. It is expressed in multiple tissues, with specific localization patterns during spermatogenesis, where acetylated H4 is found in step 11 spermatid nuclei.
  Related references: UniProt P62805.

• The protein undergoes extensive post-translational modifications, including acetylation, methylation, phosphorylation, citrullination, and various acylation events. Acetylation of lysine 5 (K5) is a well-characterized modification that influences chromatin structure and gene expression. Numerous other lysine residues (e.g., K9, K13, K17) are also subject to diverse modifications, underscoring the dynamic regulatory landscape of this histone.
  Related references: UniProt P62805.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around Lys5 of Histone H4. For optimal performance, consider using appropriate blocking buffers and detergents to minimize non-specific binding, as acetylated epitopes may be sensitive to buffer conditions.
• This antibody has been reported for use in Western blotting (WB), dot blot (DB), immunohistochemistry on paraffin sections (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. It is advisable to titrate the antibody for each specific application and sample type.
• The antibody shows predicted wide cross-reactivity across human, mouse, rat, and other species; however, we recommend validating reactivity in your specific model system prior to large-scale experiments.
• For acetylated targets, it is common to verify the specificity of detection by using deacetylase inhibitors or peptide competition assays.
• Store antibody as recommended; ship on ice bag.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined route to high-quality IVD raw materials, including validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents. Our portfolio encompasses rare and specialized targets, such as post-translationally modified histones, to support epigenetic research and assay development. Whether you need consistent supply of this Acetyl-Histone H4-K5 Rabbit mAb (P62805) or tailored sourcing solutions for other critical reagents, CamelBio bridges the gap from discovery to clinical application.