Acetyl-Histone H4-K16 Rabbit Polyclonal Antibody (P62805) for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, limiting its accessibility to cellular machineries involved in transcription, replication, repair, and chromosomal stability. Related references: UniProt P62805

H4 localizes primarily to the nucleus and chromosomes. Acetylated H4 has been shown to localize to the nucleus in specific cell types, such as spermatids during step 11 of differentiation (by similarity). Related references: UniProt P62805

Histone H4 undergoes extensive post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, citrullination, and ubiquitination. These PTMs, collectively referred to as the "histone code," dynamically regulate chromatin structure and gene expression. Related references: PMID:17967882, PMID:2474456

This antibody specifically detects acetylation at lysine 16 of histone H4 (H4K16ac), a modification associated with transcriptional activation and chromatin relaxation.

Experimental Guidance and Technical Tips

• In western blot (WB), expect a band at approximately 11 kDa for histone H4.
• For immunofluorescence (IF/ICC), co-staining with a DNA dye such as DAPI is recommended to confirm nuclear localization.
• In ELISA, consider using acetylated and non-acetylated peptide controls to assess binding specificity.
• Optimal antibody dilutions and incubation conditions should be determined empirically for each sample type and application.
• Due to high evolutionary conservation of histone H4, this antibody is predicted to cross-react with a wide range of species; verify reactivity in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop solution for IVD raw materials, covering every stage from concept to clinic. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. We invite you to explore how our acetyl-Histone H4-K16 antibody and broader histone modification portfolio can support your epigenetic research and assay development projects.