Acetyl-Histone H4-K16 Rabbit Monoclonal Antibody (P62805) for WB, ChIP, and IHC-P Applications

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Core nucleosome component: Histone H4 is a fundamental building block of the nucleosome, the basic repeating unit of chromatin. It wraps and compacts DNA, thereby regulating access to the genetic material and playing a central role in transcription, DNA repair, replication, and chromosomal stability. The dynamic state of chromatin is governed by a complex array of post-translational modifications (the "histone code") and nucleosome remodeling. Related references: UniProt P62805

• Subcellular localization: Histone H4 is predominantly localized to the nucleus and is a major constituent of chromosomes. Acetylated forms, such as those involving K16, are found in specific nuclear contexts, including the nuclei of elongating spermatids. Related references: UniProt P62805

• Post-translational modifications and K16 acetylation: Histone H4 is heavily modified, especially at lysine residues. Acetylation at lysine 16 (K16, corresponding to position 17 in the processed UniProt sequence) is a critical epigenetic mark. This modification is associated with open chromatin and transcriptionally active regions. It is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs), and is recognized by bromodomain-containing proteins to modulate gene expression. Related references: PMID:17967882, PMID:2474456, PMID:7664735, PMID:19608861

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic acetylated peptide around K16 of human Histone H4, ensuring specificity for the acetylated form. Always include appropriate controls (e.g., non-acetylated peptide competition or total Histone H4 antibody) to confirm specificity in your experimental system.
• For Western blotting (WB), a single band at approximately 11 kDa is expected. Consider optimizing loading amounts and blocking conditions to reduce nonspecific binding.
• In chromatin immunoprecipitation (ChIP), cross-linking and chromatin shearing conditions should be carefully optimized. The antibody may be suitable for both native and cross-linked ChIP; validate the recovery of known K16-acetylated genomic regions.
• For immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution will need empirical determination. The broad predicted cross-reactivity (human, mouse, rat, and others) makes this antibody a versatile tool, but staining patterns should be verified with appropriate biological controls.
• The high evolutionary conservation of Histone H4 underlies the wide predicted species reactivity. However, we recommend validating the antibody in your specific species and tissue model due to potential variations in fixation or processing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, technical services, and consultative support—from concept to clinic. This Acetyl-Histone H4-K16 rabbit monoclonal antibody exemplifies our commitment to supplying validated epigenetic tools for chromatin research. Beyond individual antibodies, we offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, all designed to accelerate your assay development and manufacturing workflows.