Acetyl-Histone H4 (K12) Rabbit Monoclonal Antibody for WB, ChIP, ChIP-seq, ELISA | P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, wrapping and compacting DNA to form chromatin. It plays a central role in regulating transcription, DNA repair, replication, and chromosomal stability. Related references: UniProt P62805
• Chromatin function is dynamically modulated by post-translational modifications of histones, such as acetylation. Acetylation at lysine 12 of histone H4 (H4K12ac) is one such mark that is associated with active chromatin and transcriptional regulation. Related references: PMID:2474456, PMID:7664735
• Histone H4 localizes to the nucleus and chromosomes, where it ensures proper chromatin architecture and epigenetic information transmission. Related references: UniProt P62805
• The human HIST2H4A gene, among others, encodes identical histone H4 proteins, reflecting the high conservation and functional importance of this histone across species. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using nuclear extracts or histones purified from cells treated with deacetylase inhibitors (e.g., TSA, nicotinamide) to enrich for acetylated H4K12. Validate signal specificity by comparing to a non-acetylated peptide-blocked control or using a pan-H4 antibody.
• In chromatin immunoprecipitation (ChIP) and ChIP-seq applications, this antibody is designed to recognize acetylated H4K12. It is advisable to include a control antibody (e.g., IgG) and to confirm the acetylation status of the target locus by independent methods such as qPCR on known positive/negative regions.
• For ELISA and dot blot (DB), coat the antigen (acetylated peptide) and test alongside the non-acetylated counterpart to ensure antibody specificity.
• Given the predicted wide cross-reactivity, this antibody may recognize the highly conserved histone H4 across many species; however, individual validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports diagnostic manufacturers and research institutes with reliable supply of high-quality antibodies like this acetyl-histone H4 (K12) rabbit mAb for epigenetics and chromatin research. We offer a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with rare target raw-material sourcing—helping you streamline assay development from concept to clinic.