Acetyl-Histone H4-K12 Rabbit mAb (P62805) for ChIP, WB, and IHC-P | CamelBio

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is one of the four core histone proteins that form the nucleosome, the fundamental repeating unit of chromatin. Along with histones H2A, H2B, and H3, it wraps and compacts DNA, thereby regulating access to the genetic material. This compaction is essential for proper chromosome segregation during cell division and for the spatial organization of the genome within the nucleus. Histone H4 plays a central role in DNA-templated processes such as transcription, replication, and repair by controlling DNA accessibility through a complex array of post-translational modifications, collectively known as the histone code.
Related references: UniProt P62805

The N-terminal tail of histone H4 is subject to extensive post-translational modifications, including acetylation, methylation, phosphorylation, and others. Acetylation at lysine 12 (H4K12ac; corresponding to residue 13 of the mature protein) is a well-characterized histone mark associated with transcriptionally active chromatin and is recognized by various bromodomain-containing proteins. This modification is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs), and its presence at specific genomic loci correlates with gene activation and open chromatin states.
Related references: PMID:17967882, PMID:2474456, PMID:7664735

Histone H4 is predominantly localized in the nucleus and is tightly associated with chromosomal DNA. In certain contexts, such as during spermatogenesis, acetylated histone H4 becomes enriched in the nucleus of elongating spermatids, suggesting a role in chromatin remodeling during germ cell development. The protein is ubiquitously expressed across tissues, reflecting its fundamental role in chromatin structure.
Related references: UniProt P62805

Experimental Guidance and Technical Tips

• For chromatin immunoprecipitation (ChIP) experiments, consider optimizing crosslinking time and sonication conditions to shear chromatin to a size range of 200–500 bp, as histone–DNA interactions are robust.
• When performing western blotting, enrichment of histones by acid extraction or histone purification kits can improve signal. Keep samples on ice and add appropriate histone deacetylase inhibitors if acetylation levels need to be preserved.
• This antibody is predicted to have wide cross-reactivity across species due to the highly conserved sequence of histone H4. Validate the antibody in the species and tissue system of interest, as acetylation patterns may vary.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate buffer and careful optimization of antibody concentration are recommended to achieve specific nuclear staining.
• The rabbit monoclonal format provides consistent lot-to-lot performance and high specificity, making it suitable for multi-sample comparisons and quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, research laboratories, and biotech institutions with a seamless one-stop sourcing experience for IVD raw materials. As your partner from concept to clinic, we offer a curated portfolio of antibodies—including validated pairs and optimized monoclonal and polyclonal antibodies—alongside bulk ancillary reagents and rare-target raw-material sourcing. For acetyl-histone H4 research and similar chromatin biology projects, CamelBio can supply this high-specificity rabbit monoclonal antibody and support your broader needs, from technical consultation to custom raw-material procurement.