Acetyl-Histone H3 (K9/K14/K18/K23/K27) Rabbit Polyclonal Antibody for WB, ELISA - Q16695/P68431

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Together with histones H2A, H2B, and H4, it wraps and compacts DNA, thereby regulating access to the genetic information. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled by a complex array of post-translational modifications, often referred to as the "histone code," and by nucleosome remodeling.

Related references: No specific reference cited for the functional role; the description is based on canonical UniProt annotation.

Acetylation of specific lysine residues on the N-terminal tail of histone H3, including K9, K14, K18, K23, and K27, is a hallmark of transcriptionally active chromatin and open chromatin structure. These modifications neutralize the positive charge of lysine, reducing the affinity between histones and DNA, and create binding platforms for reader proteins that facilitate gene activation.

Related references: PMID:16185088, PMID:16267050, PMID:16457588, PMID:16627869, PMID:17194708

Histone H3 is predominantly localized to the nucleus and chromosomes. Different H3 variants exhibit tissue-specific expression patterns; for example, the H3.1t variant (Q16695) is specifically expressed in testicular cells, while canonical H3.1 (P68431) is ubiquitously expressed. The multiple acetylation sites covered by this antibody make it a versatile tool for studying global H3 acetylation dynamics.

Related references: PMID:16185088, PMID:17194708

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates or acid-extracted histones to enrich for histone proteins. Validate the antibody specificity by treating cells with histone deacetylase inhibitors (e.g., trichostatin A) to increase global acetylation levels.
• In ELISA, employ immobilized acetylated and non-acetylated peptides to confirm binding preference for the acetylated forms.
• Due to sequence conservation across species, this antibody is predicted to cross-react with mouse, rat, and other organisms, but validation in the relevant sample system is recommended.
• As a rabbit polyclonal antibody raised against a multi-acetylated peptide, it may recognize each acetylated site with varying affinity; consider using site-specific antibodies for individual site analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing platform for high-quality IVD raw materials. From this Acetyl-Histone H3 (K9/K14/K18/K23/K27) Rabbit Polyclonal Antibody to a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, we support your entire development pipeline—from concept to clinic. Our epigenetic research reagents, including acetylated histone antibodies, are ready to streamline your ELISA and Western blot workflows with reliable, lot-to-lot consistency.