Acetyl-Histone H3-K9 Rabbit mAb (Q16695/P68431) for Epigenetics Research and ChIP Applications

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, where it helps wrap and compact DNA into chromatin. This structural role is fundamental for regulating DNA accessibility to cellular machinery involved in transcription, DNA repair, replication, and chromosomal stability.
  Related references: UniProtKB Q16695, UniProtKB P68431

• Post-translational modifications (PTMs) of histone H3, including acetylation, methylation, phosphorylation, citrullination, and others, constitute a dynamic “histone code” that modulates chromatin structure and gene expression.
  Related references: UniProtKB Q16695, UniProtKB P68431

• Acetylation occurs at multiple lysine residues, such as K5, K10, K15, K19, K24, K28, and K37, among others. This antibody specifically recognizes acetylated lysine 9 (K9) of histone H3. While K9 acetylation is not explicitly annotated in the UniProt records for Q16695 and P68431, it is a known modification associated with active chromatin regions.
  Related references: For histone acetylation evidence, see PMID:16185088 (K10 acetylation), PMID:16267050 (multiple acetylation sites)

• Histone H3 localizes to the nucleus and is a major chromosomal protein.
  Related references: UniProtKB Q16695, UniProtKB P68431

• Multiple gene copies encode histone H3 proteins. Q16695 (H3-4, HIST3H3) corresponds to the testis-specific H3.1t variant, expressed predominantly in testicular cells. P68431 represents the widely expressed H3.1 isoform, encoded by several genes such as H3C1 (HIST1H3A) and H3C2 (HIST1H3B).
  Related references: UniProtKB Q16695, UniProtKB P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide corresponding to the region around K9 of human Histone H3. This design ensures high specificity for the acetylated form; consider including non-acetylated peptide controls in your experiments to confirm specificity.
• For Western blotting, a band at approximately 15 kDa is expected. Use proper loading controls and verify expression in your sample system, as histone H3 levels may vary across cell types and conditions.
• This antibody is suitable for a wide range of applications including ChIP-seq and CUT&Tag. For chromatin-based assays, optimization of crosslinking and immunoprecipitation conditions is recommended, especially across different species and tissue types.
• In immunohistochemistry or immunofluorescence, ensure adequate permeabilization and antigen retrieval, as histones are nuclear. Validate staining patterns using known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For epigenetic research and assay development targeting acetylated histone H3-K9, we offer a wide selection of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing tailored to your needs. Contact our team to explore custom supply solutions and accelerate your project from discovery to commercialization.