Acetyl-Histone H3-K56 Rabbit Polyclonal Antibody (Q16695/P68431) for Epigenetics Research | WB, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This fundamental role regulates transcription, DNA repair, replication, and chromosomal stability through a complex set of post-translational modifications known as the histone code. Related references: UniProt Q16695

The canonical somatic histone H3.1 (SWISS P68431) is encoded by a multigene family (H3C1–H3C12) and ubiquitously expressed, while the testis-specific variant H3.1t (SWISS Q16695, gene H3-4/HIST3H3) is restricted to testicular cells.

Acetylation of lysine residues, including K56, is a pivotal epigenetic modification that alters chromatin structure and recruits effector proteins. Hyperacetylation generally correlates with transcriptional activation, while deacetylation leads to gene silencing. Related references: PMID:17194708

Subcellular localization is exclusively nuclear, associated with chromosomes during cell division and dispersed throughout the nucleus during interphase.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K56; consider using acetylation-deficient mutants or deacetylase inhibitors to confirm specificity in your experimental system.
• For Western blot, expect a band at ~15 kDa. Nuclear fractionation or acid extraction of histones is recommended for clear detection.
• In ChIP assays, optimize cross-linking time and antibody amount per reaction. Include well-characterized positive (e.g., active promoters) and negative (heterochromatic) genomic regions as controls.
• Broad predicted cross-reactivity necessitates validation of reactivity in the species and tissue of interest before proceeding with large-scale experiments.
• The antibody is listed for multiple applications (WB, IF/ICC, ELISA, ChIP); independent optimization and validation are advised for each technique.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform for IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. Our Acetyl-Histone H3-K56 Rabbit pAb supports epigenetic studies and diagnostic development targeting histone modifications. Whether you need rare-target antibody sourcing or scalable production, CamelBio bridges your concept to clinic with reliable supply solutions.