Acetyl-Histone H3-K56 (Q16695 / P68431) Rabbit Monoclonal Antibody for ChIP, WB, IF

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Together with histones H2A, H2B, and H4, it forms an octamer around which DNA is wrapped, compacting the genome and regulating access for transcription, replication, and repair. This architecture underpins chromosomal stability and the dynamic control of gene expression.

Related references:

• UniProtKB Q16695

• UniProtKB P68431

• Histone H3 is subject to an extensive array of post-translational modifications (PTMs), often termed the "histone code." These include acetylation, methylation, phosphorylation, citrullination, and many others, which collectively modulate chromatin structure and effector protein recruitment. Acetylation at lysine 56 (K56) is a particularly notable mark implicated in nucleosome assembly, DNA damage response, and transcriptional regulation.

Related references:

• PMID:17194708

• PMID:19608861

• Histone H3 localizes exclusively to the nucleus and associates with chromosomes during cell division. The canonical H3.1 variant (encoded by multiple genes including HIST1H3A, SWISS P68431) is expressed replication-dependently throughout the cell cycle, while the H3.1t variant (Q16695) is testis-specific and contributes to chromatin organization in male germ cells.

Related references:

• UniProtKB Q16695

• UniProtKB P68431

• The antibodies against acetylated K56 are valuable tools for probing DNA replication-coupled chromatin assembly, genotoxic stress pathways, and the dynamics of epigenetic inheritance. The acetyl-specific immunogen used to generate this antibody ensures high selectivity for the modified form over unmodified H3.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to acetylated K56 of human Histone H3. This design should confer high specificity; however, researchers may consider verifying selectivity by comparing signals between acetylated and non-acetylated peptide-blocked controls in their experimental systems.
• The antibody is recommended for a broad range of applications including ChIP-seq, CUT&Tag, and IF. For chromatin-based assays, optimize cross-linking and sonication conditions to preserve the K56 acetylation epitope. In immunofluorescence, a nuclear staining pattern is expected.
• The predicted protein weight is 15 kDa. When running WB, include appropriate molecular weight markers and consider using a histone extraction protocol to enrich for nuclear proteins.
• Although cross-reactivity is predicted across a wide range of species, it is advisable to validate performance in non-human samples by sequence alignment around the acetylation site or by testing in the relevant biological material.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for diagnostic manufacturers and research laboratories, offering a full portfolio of IVD raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This Acetyl-Histone H3-K56 rabbit monoclonal antibody fits seamlessly into epigenetics and chromatin biology workflows, and our team can assist with scaling supply or identifying complementary reagents to accelerate your assay development from concept to clinic.