Acetyl-Histone H3-K27 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, DB, ELISA, ChIP – CamelBio

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, thereby controlling DNA accessibility for transcription, replication, and repair. Histones play a central role in maintaining chromosomal stability and regulating gene expression through a dynamic array of post-translational modifications (PTMs) known as the "histone code." These modifications, including acetylation, methylation, phosphorylation, and ubiquitination, orchestrate chromatin structure and recruit effector proteins.

The H3 variants, such as the canonical H3.1 (encoded by multiple genes including H3C1–H3C12) and the testis-specific H3.1t (H3-4), share highly conserved sequences. Acetylation at lysine 27 (H3K27ac) is a key epigenetic mark primarily associated with active enhancers and promoters, facilitating an open chromatin conformation permissive to transcription.

Histone H3 localizes predominantly to the nucleus and is found along chromosomes. While H3.1 is expressed in most dividing cells, H3.1t is enriched in testicular cells, reflecting its role in chromatin remodeling during spermatogenesis.

Related references: PMID:17194708

Experimental Guidance and Technical Tips

• For Western blotting (WB) , consider using whole cell lysates or histone-enriched extracts. Confirm specificity by pre-incubating the antibody with acetylated versus non-acetylated blocking peptides.
• In dot blot (DB) and ELISA applications, titrate the antibody and include both acetylated and non-acetylated peptide controls to assess binding specificity.
• For ChIP, optimize crosslinking and sonication conditions for your cell type. Validate enrichment by qPCR at known H3K27ac-positive loci (e.g., active gene promoters) and negative control regions. Always include an IgG mock control.
• General tip: Because histone modifications are dynamic and can change during the cell cycle or in response to stimuli, always handle and process samples consistently to preserve PTMs and avoid artifacts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop access to IVD raw materials and ancillary supplies. Beyond this high-quality rabbit polyclonal antibody against acetyl-Histone H3-K27, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare epigenetic targets. Whether you need routine bulk orders or support for custom assay development, CamelBio’s integrated supply chain and technical consultation are designed to accelerate your IVD projects from concept to clinic.