Acetyl-Histone H3-K27 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, ChIP-seq, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin. Nucleosomes wrap and compact DNA, restricting access to the DNA template for transcription, replication, repair, and other cellular processes. Histone H3 plays a central role in maintaining chromosomal stability and regulating gene expression through a complex interplay of post-translational modifications, collectively referred to as the histone code. Nucleosome remodeling further modulates chromatin accessibility.

Related references: UniProt Q16695, UniProt P68431

Consistent with its role, Histone H3 is predominantly localized in the nucleus, where it associates with chromosomal DNA to form chromatin fibers.

Related references: UniProt Q16695, UniProt P68431

Among the multiple Histone H3 variants, H3.1t (encoded by H3-4/HIST3H3, UniProt Q16695) exhibits testis-specific expression, while the canonical H3.1 (encoded by a cluster of genes, UniProt P68431) is ubiquitously expressed.

Related references: UniProt Q16695

Histone H3 is subject to an extensive array of post-translational modifications (PTMs) on its flexible N-terminal tail, including acetylation, methylation, phosphorylation, citrullination, ADP-ribosylation, and others. These PTMs act in a combinatorial fashion to dictate chromatin dynamics and regulate DNA-templated processes. The acetylation status of specific lysine residues, such as K27, is a critical determinant of active regulatory regions, with H3K27ac serving as a hallmark of active promoters and enhancers. This product specifically recognizes the acetylated form of Lys27.

Related references: UniProt P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around Lys27 of human Histone H3 (NP_003520.1). This design facilitates specific recognition of the acetylated state; however, potential cross-reactivity with non-acetylated H3 or other acetylated histones should be evaluated in the relevant sample system.
• Based on listed applications, this antibody is suitable for Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), immunoprecipitation (IP), ELISA, and chromatin immunoprecipitation (ChIP, ChIP-seq). For ChIP-seq, consider optimizing antibody concentration and crosslinking conditions.
• The predicted molecular weight of Histone H3 is approximately 15 kDa; distinct bands in this region are expected in WB analysis with an appropriate nuclear preparation.
• Wide species cross-reactivity (Human, Mouse, Rat, Other) is predicted due to high sequence conservation; users should verify reactivity in their specific model organism.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, connecting diagnostic developers and researchers with reliable, high-quality antibodies and ancillary reagents. From validated antibody pairs to customized bulk orders, we supply essential tools for chromatin biology and epigenetic research, exemplified by this acetyl-Histone H3-K27 antibody. Reach out to explore how our one-stop procurement services can accelerate your assay development pipeline.