Acetyl-Histone H3-K27 Rabbit Monoclonal Antibody (Q16695 / P68431) for WB, IHC-P, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin, thereby regulating DNA accessibility to the cellular machinery. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. A complex set of post-translational modifications (PTMs) on histones, collectively known as the histone code, dynamically controls chromatin structure and function.
Related references:
PMID:16627869
PMID:17194708

Histone H3 has several variants; H3.1 (encoded by genes such as H3C1-H3C12, UniProt P68431) is the canonical replication-dependent form expressed throughout the cell cycle, while H3.1t (H3-4, UniProt Q16695) is a testis-specific variant. Both localize to the nucleus and are integral parts of chromosomes.
Related references:
UniProt Q16695
UniProt P68431

Acetylation at lysine 27 of histone H3 (H3K27ac) is a well-characterized mark of active enhancers and promoters, distinguishing them from poised or inactive regulatory elements. This modification, catalyzed by histone acetyltransferases such as p300/CBP, promotes an open chromatin conformation and facilitates transcriptional activation. Dysregulation of H3K27 acetylation has been implicated in cancer and other diseases.
Related references:
PMID:16627869
PMID:17194708

Experimental Guidance and Technical Tips

• When detecting acetylation, include deacetylase inhibitors (e.g., sodium butyrate, TSA) in lysis buffers to preserve endogenous acetylation levels.
• Use whole-cell lysates from HeLa, HEK293, or other appropriate cell lines as positive controls; induced or treated samples may exhibit enhanced H3K27ac signal.
• For ChIP assays, ensure that the antibody specifically enriches H3K27ac-marked chromatin; consider cross-linking conditions and sonication optimization.
• Confirm antibody specificity using an acetylated peptide blocking experiment or by comparing signals with a pan-H3 antibody.
• This antibody is suitable for WB, IHC-P, IF/ICC, IP, ELISA, DB, and ChIP; validate performance in the relevant sample system for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For epigenetic and chromatin research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need this acetyl-Histone H3-K27 antibody or other specialized reagents, CamelBio is committed to bridging your supply chain needs with high-quality, reliable IVD components.