Acetyl-Histone H3-K23 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, ChIP, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of nucleosomes, the fundamental repeating units of chromatin. Nucleosomes wrap and compact DNA, restricting access to the cellular machineries that require DNA as a template. Through this structural role, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. Accessibility is dynamically modulated by a complex array of post-translational modifications (PTMs) collectively known as the histone code, along with nucleosome remodeling.

Acetylation at lysine 23 (K23) of the histone H3 N-terminal tail is a significant PTM that contributes to chromatin decondensation and gene activation. This site corresponds to K24 in some sequence numbering due to initiator methionine cleavage. Multiple studies have identified K23 acetylation in various cellular contexts, linking it to open chromatin states and transcriptional regulation.

Related references: PMID:19608861, PMID:16267050, PMID:16457588

The Histone H3.1 protein (encoded by genes such as H3C1, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3C10, H3C11, H3C12, and HIST1H3A) is typically localized to the nucleus and chromosome. The testis-specific H3.1t isoform (Q16695) is expressed in testicular cells, while the canonical H3.1 (P68431) is ubiquitous. Both variants share the conserved K23 acetylation site.

Histone H3 is subject to numerous additional PTMs, including methylation, phosphorylation, citrullination, and other acylations, which intricately regulate chromatin function. The antibody’s specificity for the acetylated K23 form enables precise dissection of these regulatory mechanisms.

Experimental Guidance and Technical Tips

• This antibody is generated against a synthetic acetylated peptide corresponding to residues surrounding K23 of human Histone H3. Cross-reactivity is predicted across a wide range of species due to sequence conservation of this region; always confirm with your own samples.
• For Western blot (WB), a band around 15 kDa is expected. Consider loading appropriate positive and negative controls (e.g., cells treated with histone deacetylase inhibitors vs. untreated) to verify acetylation-dependent signal.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of antigen retrieval and blocking may be required; validate specificity using peptide competition or knockout cell lines if available.
• For chromatin immunoprecipitation (ChIP), ensure proper cross-linking and shearing. Include a non-specific IgG control and test different antibody concentrations. This antibody is suitable for ChIP as indicated by the supplier.
• ELISA and other applications should be validated in the relevant sample system. Sensitivity and specificity may vary between assays.
• As with any site-specific modification antibody, batch-to-batch consistency may affect results; always titrate and confirm the optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This acetyl-Histone H3-K23 antibody is ideal for epigenetic research and assay development projects. Whether you need bulk supplies, rare target antibody sourcing, or complete raw-material solutions, CamelBio provides a seamless procurement experience from concept to clinic.