Acetyl-Histone H3-K18 Rabbit Monoclonal Antibody (Q16695/P68431) | Validated for WB, IHC, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core nucleosomal protein that wraps and compacts DNA into chromatin, limiting accessibility to DNA-dependent machineries and playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProtKB Q16695, UniProtKB P68431

• It localizes to the nucleus and associates with chromosomes. Related references: UniProtKB Q16695, UniProtKB P68431

• The canonical H3.1 is encoded by multiple genes (e.g., H3C1, H3C2) and is expressed in a replication-dependent manner in somatic cells. The H3.1t variant (Q16695) is specifically expressed in testicular cells. Related references: UniProtKB Q16695

• Histone H3 undergoes extensive post-translational modifications that constitute the "histone code." Acetylation at K18 (position 19 in the canonical H3 sequence) is a key mark linked to transcriptional activation and DNA repair. This modification is dynamically regulated by HATs and HDACs. Acetylation at this site has been documented in multiple studies. Related references: PMID:19608861, PMID:16267050, PMID:16627869, PMID:17194708, PMID:35939806

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K18 of human Histone H3. The resulting rabbit monoclonal antibody is expected to specifically recognize acetylated H3K18, with no cross-reaction to unmodified K18 or other acetylation sites. Validate specificity using both acetylated and non-acetylated peptide competition assays or by treating cells with HDAC inhibitors.
• For Western blotting (WB), a single band at ~16 kDa is anticipated. Use appropriate loading controls (e.g., total Histone H3 or H3 C-terminal antibodies) to normalize acetylation levels. Acetylation signals may be enhanced by treating cells with deacetylase inhibitors such as trichostatin A (TSA) or sodium butyrate.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions. Endogenous peroxidase quenching is recommended for IHC-P. Validate staining by comparing with isotype controls.
• For chromatin immunoprecipitation (ChIP), ensure cross-linking conditions are optimized for histone modifications. Use appropriate positive and negative control loci (e.g., actively transcribed genes vs. silenced loci). Enrichment efficiency can be verified by qPCR.
• Due to wide predicted cross-reactivity (human, mouse, rat, and others), confirm reactivity in the species of interest before proceeding with large-scale experiments. Sequence alignment shows high conservation of the region surrounding K18 across vertebrates.
• For ELISA and dot blot (DB) applications, the antibody can be used to detect acetylated H3K18 peptides or recombinant histones. Consider using a biotinylated secondary antibody and streptavidin-HRP for signal amplification.

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CamelBio is a premier one-stop supplier of IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinical application. We offer a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. For your epigenetics and chromatin research needs, including acetylated histone antibodies like this Anti-Acetyl-Histone H3-K18 rabbit mAb, CamelBio provides reliable procurement and dedicated support to accelerate your assay development and biomarker discovery.