Acetyl-Histone H3-K18 (Q16695/P68431) Rabbit Polyclonal Antibody for WB, IHC-P, ChIP and ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of nucleosomes, which wrap and compact DNA into chromatin, thereby regulating DNA accessibility to the cellular machinery. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is governed by the “histone code”—a complex set of post-translational modifications (PTMs) including acetylation, methylation, phosphorylation, and others, along with nucleosome remodeling. Related references: UniProt: Q16695, P68431

Histone H3 localizes primarily to the nucleus and is associated with chromosomes. Related references: UniProt: Q16695, P68431

The H3.1t isoform (Q16695) is predominantly expressed in testicular cells, while the canonical H3.1 (P68431) is widely expressed. Related references: UniProt: Q16695

Acetylation at lysine 18 (K18, corresponding to position 19 in UniProt numbering due to initiator methionine cleavage) is a well-characterized post-translational modification associated with active chromatin and transcriptional regulation. Mass spectrometry and biochemical studies have confirmed K18 acetylation in human cells. Related references: PMID:19608861, PMID:16267050, PMID:16627869, PMID:17194708

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using histone extraction protocols for optimal detection. The predicted molecular weight of histone H3 is approximately 15 kDa.
• For immunohistochemistry (IHC-P), antigen retrieval may enhance staining. Validate in tissue samples known to express acetylated H3-K18.
• For chromatin immunoprecipitation (ChIP), this antibody can be used to enrich chromatin regions marked by H3K18ac; cross-linking conditions and antibody concentration should be optimized in the relevant cell system.
• When using for ELISA, pairing with a suitable detection antibody is recommended. Validate coating conditions and peptide competition to ensure specificity.
• The antibody shows broad cross-reactivity predicted among human, mouse, and rat, making it suitable for translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This Acetyl-Histone H3-K18 antibody serves as a critical tool for epigenetic-based assay development and chromatin research. Beyond premium antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing of rare target reagents to streamline your commercial IVD kit production.