Acetyl-Histone H3-K18 (P68431/Q16695) Rabbit mAb | Western Blot, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of nucleosomes, serving as structural elements that wrap and compact DNA into chromatin, limiting DNA accessibility to cellular machineries. This plays a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Related references: UniProt
• Histone H3 localizes primarily to the nucleus and chromosome.
• The N-terminal tails of histones undergo extensive post-translational modifications (PTMs), collectively termed the "histone code", which regulate chromatin structure and function. Acetylation of specific lysine residues, such as lysine 18 (K18), is associated with active transcription and is recognized by bromodomain-containing proteins. Related references: PMID:16267050, PMID:16627869
• The two SWISS accession numbers represent distinct histone H3 variants: P68431 (canonical H3.1) is widely expressed, while Q16695 (H3.1t/H3.4) is testis-specific. Both share high sequence identity, and the antibody raised against the acetylated peptide around K18 of P68431 may cross-react with other H3 variants due to conserved sequences. Related references: UniProt Q16695

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is approximately 15 kDa; optimize loading amounts and antibody dilution based on your sample’s histone expression levels.
• For chromatin immunoprecipitation (ChIP, ChIP-seq) and CUT&Tag, this antibody recognizes endogenous acetylated histone H3; include appropriate positive and negative controls (e.g., transcriptionally active vs. silent loci) to confirm specificity.
• When using for dot blot (DB) or ELISA, consider pairing with an unmodified H3 antibody to normalize signals.
• Cross-reactivity is predicted to be wide-range; validate reactivity in species other than human, mouse, and rat if required.
• All experimental conditions should be validated in the relevant sample system under your laboratory conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Acetyl-Histone H3-K18 Rabbit mAb is part of our extensive portfolio of epigenetic antibodies and reagents, ideal for chromatin biology and biomarker discovery. We offer a wide range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and scale-up needs.