Acetyl-Histone H3-K14 Rabbit Monoclonal Antibody (Q16695 / P68431) for WB, DB, ChIP, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, which wraps and compacts DNA into chromatin, thereby regulating DNA accessibility for transcription, replication, repair, and maintaining chromosomal stability. This role is essential for all DNA-templated processes. Related references: PMID:16185088, PMID:16267050
• The H3 isoforms H3.1 (encoded by multiple gene copies, e.g., HIST1H3A-J) and H3.1t (testis-specific, encoded by HIST3H3) share the same histone fold domain and are primarily localized to the nucleus and chromosomes. H3.1t is specifically expressed in testicular cells, while canonical H3.1 is broadly expressed.
• Post-translational modifications of histones, especially acetylation, methylation, phosphorylation, and others, constitute the "histone code" that governs chromatin dynamics. Acetylation at lysine 14 of histone H3 (H3K14ac) is a hallmark of active chromatin and is recognized by bromodomain-containing proteins. Related references: PMID:16267050, PMID:16627869
• The antibody specifically targets the acetylated form of K14, enabling the study of H3K14ac-mediated transcriptional regulation and epigenetic marks.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K14 of human Histone H3. When designing similar antibodies, consider using an acetylated peptide to ensure specificity for the acetylated form.
• For Western blotting (WB), validate the antibody using whole cell lysates from human, mouse, or rat cells. Treatment with histone deacetylase inhibitors (e.g., TSA or sodium butyrate) may enhance the signal and confirm acetylation-dependent detection.
• In chromatin immunoprecipitation (ChIP), standard crosslinking and shearing protocols are suitable. Including appropriate controls (e.g., non-acetylated peptide competition, normal rabbit IgG) is recommended to ensure ChIP-grade specificity.
• For ELISA and dot blot (DB) applications, use acetylated versus non-acetylated H3K14 peptides as positive and negative controls to verify binding.
• Cross-reactivity with mouse and rat has been observed; however, users should validate the antibody in their specific experimental system, as target expression and modification levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For scientists investigating histone modifications and chromatin biology, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, facilitating efficient assay development and scalable production. Partner with us to streamline your sourcing of high-quality raw materials for epigenetic research and in vitro diagnostics.