Acetyl-Histone H2B-K5 Rabbit Polyclonal Antibody (P62807/Q16778) for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. Together with histones H2A, H3, and H4, it wraps and compacts DNA, thereby regulating DNA accessibility. This plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProtKB P62807, UniProtKB Q16778

Post-translational modifications (PTMs) of histones, including acetylation, methylation, phosphorylation, and ubiquitination, constitute the "histone code" that dictates chromatin dynamics and gene expression. Acetylation of specific lysine residues, such as K5 on histone H2B, neutralizes the positive charge of the histone tail, relaxing chromatin structure and facilitating transcriptional activation. This modification is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs). Related references: PMID:16283522, PMID:16627869

Histone H2B is predominantly localized in the nucleus, associated with chromosomes. It is expressed in multiple variant forms, encoded by different gene clusters, but shares high sequence conservation across species. Beyond its nuclear roles, H2B exhibits broad antibacterial activity and may contribute to the antimicrobial barrier of colonic epithelium and amniotic fluid. Related references: UniProtKB P62807, UniProtKB Q16778

The SWISS accessions P62807 (Histone H2B type 1-C/E/F/G/I) and Q16778 (Histone H2B type 2-E) represent two of several human histone H2B variants, all sharing the conserved K5 acetylation site targeted by this antibody.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic acetylated peptide corresponding to K5 of human histone H2B. It is expected to specifically recognize the acetylated form; validate in your sample system using appropriate positive and negative controls (e.g., samples treated with HDAC inhibitors vs. untreated).
• For Western blot (WB), consider using a nuclear-enriched lysate and standard SDS-PAGE conditions. A band around 14 kDa (the approximate molecular weight of histone H2B) should be detected.
• For IHC-P, IF/ICC, and ELISA, optimize antibody dilution and include antigen retrieval steps as needed. Since the immunogen is a short peptide, antigen retrieval may be critical for IHC.
• The antibody is predicted to cross-react widely across human, mouse, rat, and other species due to the highly conserved sequence around K5. However, cross-reactivity should be confirmed experimentally in each species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with comprehensive access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This Acetyl-Histone H2B-K5 Rabbit Polyclonal Antibody exemplifies our commitment to supplying high-quality tools for epigenetics and chromatin research. Whether you need rare target antibodies, enzyme conjugates, or custom reagent sourcing, CamelBio offers a seamless procurement experience to accelerate your development from concept to clinic.