Acetyl-Histone H2B-K15 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA | P62807/Q16778

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

• Histone H2B is a core component of nucleosomes, compacting DNA into chromatin and thereby regulating transcription, DNA repair, replication, and chromosomal stability. This role is orchestrated through a complex array of post-translational modifications often referred to as the histone code.

• Beyond its nuclear functions, Histone H2B possesses broad antibacterial activity and contributes to the antimicrobial barriers of the colonic epithelium and amniotic fluid.

• Histone H2B predominantly localizes to the nucleus and chromosomes, where it is assembled into nucleosomes.

• The two major variants represented by SWISS accessions P62807 (Histone H2B type 1-C/E/F/G/I) and Q16778 (Histone H2B type 2-E) share high sequence similarity and are subject to numerous PTMs, including acetylation, methylation, phosphorylation, and crotonylation. Lysine 15 is a conserved residue across isoforms and is a known site for regulatory modifications; this antibody specifically recognizes the acetylated form at that position.

Related references: UniProt P62807, UniProt Q16778

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates or nuclear extracts. The expected molecular weight is approximately 14 kDa based on sequence analysis.
• When performing IHC-P or IF/ICC, antigen retrieval and fixation conditions may influence the detection of acetylation-specific epitopes.
• In ELISA, diluted serum or cell culture supernatants can be tested; validate sensitivity with purified acetylated peptide controls.
• Acetylation-specific antibodies may exhibit background; consider blocking with BSA and using appropriate secondary antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including optimized antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. Leveraging our extensive supplier network, we source rare targets such as post-translationally modified histones to accelerate your assay development from concept to clinic. Our catalog includes high-quality Histone H2B antibodies suitable for epigenetics research and clinical diagnostics.