Acetyl-Histone H2B-K12 Rabbit pAb (P62807/Q16778) for WB, IHC-P, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

• Core component of nucleosome; wraps and compacts DNA into chromatin, limiting accessibility to cellular machineries. Histone H2B plays a central role in transcription regulation, DNA repair, replication, and chromosomal stability. DNA accessibility is governed by a complex set of post-translational modifications, known as the histone code, and nucleosome remodeling. Related references: UniProtKB P62807, UniProtKB Q16778
• Also exhibits broad antibacterial activity and may contribute to the functional antimicrobial barrier of the colonic epithelium and the bactericidal activity of amniotic fluid. Related references: UniProtKB P62807, UniProtKB Q16778
• Subcellular localization: Nucleus and chromosome. Related references: UniProtKB P62807, UniProtKB Q16778
• Undergoes extensive post-translational modifications, including acetylation, methylation, phosphorylation, crotonylation, and others. Acetylation at lysine 12 (K12) is a well-characterized modification involved in epigenetic regulation and chromatin dynamics. Related references: PMID:16627869

Experimental Guidance and Technical Tips

• This antibody is raised against an acetylated peptide around K12; verify specificity in your experimental system using appropriate controls (e.g., peptide competition or mutant cell lines).
• For WB, a typical starting dilution must be optimized. Consider loading histone-enriched extracts and using appropriate secondary detection.
• In IHC-P, antigen retrieval methods may influence epitope accessibility; test citrate- versus EDTA-based buffers.
• For ChIP, validate antibody performance in pilot pull-down assays and carefully optimize cross-linking conditions.
• Acetylation is dynamic; treat cells with deacetylase inhibitors (e.g., TSA, sodium butyrate) to enhance signal if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This acetyl-H2B-K12 rabbit polyclonal antibody is suitable for epigenetic and chromatin research applications; our supply chain can support your scale-up needs from concept to clinic. Contact our team for personalized sourcing solutions.