Acetyl-Histone H2A (K5) Rabbit Polyclonal Antibody (P04908/P0C0S8) for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2A Function and Localization

• Histone H2A is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This limits DNA accessibility and plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• The subcellular localization of H2A is primarily the nucleus and chromosome.
• Histone H2A is subject to extensive post-translational modifications (PTMs) on its N-terminal tail, including acetylation, methylation, phosphorylation, and ubiquitination. These modifications constitute the "histone code" and regulate chromatin dynamics.
• Acetylation at lysine 5 (H2AK5ac) is a key epigenetic mark associated with active transcription and open chromatin. This antibody is raised against a synthetic acetylated peptide around K5 and specifically recognizes the acetylated form.
• Multiple genes encode H2A variants (e.g., H2AC4, H2AC8, H2AC11, H2AC15, H2AC16, H2AC17), which are highly conserved across species. The immunogen sequence is derived from human H2A and is expected to cross-react with orthologs due to high sequence homology.

Related references: UniProt P04908 and UniProt P0C0S8 comprehensively annotate histone H2A functions and modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K5. To confirm specificity for the acetylated form, consider using non-acetylated peptide blocking or comparing signals from cells treated with histone deacetylase inhibitors (e.g., trichostatin A, sodium butyrate) versus untreated controls.
• In western blot (WB), a band at approximately 14 kDa is expected for Histone H2A. Signal intensity will depend on the acetylation status of the sample.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions. Methanol fixation or Triton X-100 permeabilization are typically effective for nuclear histone targets.
• This antibody has been validated for ELISA and may be suitable for quantitative assays when paired with appropriate standards.
• Reported cross-reactivity includes human, mouse, and rat, with a wide range of other species predicted based on sequence homology. Validate reactivity in your specific model system if not previously tested.
• As a polyclonal antibody, lot-to-lot variation can occur. Titration and optimization for each application and sample type are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, research institutes, and clinical laboratories worldwide. This Acetyl-Histone H2A-K5 rabbit polyclonal antibody exemplifies our commitment to supplying high-quality, application-validated reagents for epigenetic and chromatin biology research. Beyond individual antibodies, CamelBio offers a full suite of raw materials—including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or difficult-to-obtain targets. Partner with CamelBio to streamline your IVD development from concept to clinic with reliable, cost-effective procurement solutions.