Acetyl-Histone H1.4-K26 Rabbit Polyclonal Antibody (P10412) | IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H1.4 Function and Localization

• Histone H1.4 (gene H1-4, also known as HIST1H1E or H1F4) is a member of the linker histone H1 family that binds to nucleosomal DNA, facilitating higher-order chromatin folding and fiber compaction. Related references: PMID:35581345, PMID:40240600, PMID:40516528
• It plays a critical role in transcriptional regulation by modulating nucleosome spacing, chromatin remodeling, and DNA methylation. Additionally, H1.4 promotes the deposition of the repressive histone mark H3K27me3 by the PRC2 complex. Related references: PMID:35581345, PMID:40240600
• Subcellularly, Histone H1.4 localizes predominantly to the nucleus, enriched in heterochromatin regions. It distributes throughout the nucleus in a punctuate staining pattern. Related references: PMID:38530350, PMID:40240600, PMID:15911621, PMID:21852232
• Lysine 26 (K26) is a hotspot for diverse post-translational modifications, including acetylation, mono‑, di‑, and trimethylation, as well as benzoylation, underscoring its importance in epigenetic signaling. Related references: Acetylation: PMID:15469825; Methylation: PMID:19144645, PMID:3782055, PMID:30156264; Benzoylation: PMID:30154464

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic acetylated peptide around K26. For IHC-P, consider heat-induced antigen retrieval in citrate or EDTA buffer, as histone epitopes may be masked by formalin cross-linking.
• In IF/ICC, co-stain with a nuclear counterstain (e.g., DAPI) and use Triton X-100 permeabilization to confirm nuclear localization.
• For ELISA, validate specificity by comparing binding to acetylated versus non-acetylated peptide controls to confirm acetylation-dependent recognition.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be monitored; include a positive control such as HeLa whole cell lysate in each run.
• Predicted cross-reactivity with human, mouse, and rat based on sequence homology—please verify in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, providing comprehensive access to high-quality antibodies, validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-acetyl-Histone H1.4-K26 antibody supports epigenetics and chromatin research, and CamelBio can assist in securing complementary reagents for your diagnostic development pipeline, from concept to clinic. Contact us for flexible supply solutions tailored to your project needs.