Acetyl-DNMT1 (K1127/K1129/K1131/K1133) Rabbit Polyclonal Antibody (P26358) for IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: DNA (cytosine-5)-methyltransferase 1 Function and Localization

DNMT1 is a critical DNA methyltransferase responsible for maintaining CpG methylation patterns during DNA replication, ensuring epigenetic inheritance. It preferentially methylates hemimethylated DNA and associates with replication foci in S phase, but also acts independently of replication during G2 and M phases to preserve global methylation landscapes. DNMT1-mediated silencing involves direct interaction with HDAC2 and formation of corepressor complexes that modulate histone modifications and gene expression. Related references: PMID:17200670, PMID:18754681, PMID:21745816, PMID:26070743, PMID:10888872, PMID:24623306

The enzyme localizes predominantly to the nucleus and is recruited to chromatin at replication foci via its RFTS domain, which recognizes ubiquitinated histone H3. It also localizes to perinucleolar regions, reflecting specialized functions in heterochromatin maintenance. Related references: PMID:12145218, PMID:24492612, PMID:29053958

DNMT1 is ubiquitously expressed, with particularly high levels in fetal tissues, heart, kidney, placenta, and peripheral blood mononuclear cells, and lower expression in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Related references: PMID:10325416

Post-translational modifications of DNMT1, especially acetylation, play key regulatory roles. The lysine cluster targeted by this antibody (K1127/K1129/K1131/K1133 in isoform 1) corresponds to known acetylation sites (K1111/K1113/K1115/K1117/K1119/K1121 in the canonical sequence) that are modified by histone acetyltransferases and can influence protein stability, localization, and interaction with other epigenetic factors. Related references: PMID:21947282, PMID:19608861

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic acetylated peptide encompassing K1127, K1129, K1131, and K1133 of human DNMT1. It is expected to specifically recognize the acetylated form of the protein.
• For IHC-P, standard antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) may be required to expose the epitope. Optimal antibody dilution and incubation conditions should be determined empirically.
• When performing IF/ICC, fixation with 4% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 is commonly used; however, these steps must be validated in the specific cell or tissue system.
• ELISA can be used to detect and quantify acetylated DNMT1 levels in cell lysates or tissue extracts. Consider employing a peptide competition assay to confirm binding specificity.
• As DNMT1 is widely expressed, include appropriate positive and negative controls (e.g., tissues or cell lines with known acetylation status) to verify staining patterns.
• The predicted molecular weight is 183 kDa; if repurposing the antibody for unvalidated applications (e.g., Western blot), validate the band size and specificity accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a partner in advancing epigenetics research and diagnostic development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies (including acetylated-specific reagents like this anti-acetyl-DNMT1 pAb), bulk ancillary reagents, and custom sourcing for rare targets. Our supply chain expertise ensures you receive consistent, high-quality raw materials to accelerate your diagnostic and research workflows.