ABHD5 Rabbit pAb - Q8WTS1

Core Product Specifications and Parameters

Biological Background: ABHD5 Function and Localization

• ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase that converts lysophosphatidic acid to phosphatidic acid, playing a central role in phospholipid biosynthesis (PubMed:18606822). Related references: PMID:18606822
• It preferentially utilizes 1-oleoyl-LPA and arachidonoyl-CoA substrates, facilitating the production of phosphatidic acid for signaling and membrane dynamics (PubMed:18606822). Related references: PMID:18606822
• ABHD5 regulates cellular triacylglycerol storage by directly activating the rate-limiting lipase PNPLA2/ATGL, a key step in lipid droplet catabolism (PubMed:16679289). Related references: PMID:16679289
• The protein is required for normal keratinocyte differentiation, contributing to epidermal homeostasis and barrier function (PubMed:18832586). Related references: PMID:18832586
• ABHD5 localizes to lipid droplet surfaces and the cytoplasm/cytosol, where it coordinates neutral lipid turnover and droplet fusion.
• It is ubiquitously expressed, with pronounced levels in liver, skeletal muscle, brain, skin, lymphocytes, and dermal fibroblasts.
• Post-translational modifications, including acetylation and phosphorylation, fine-tune its activity, and loss-of-function mutations are associated with Chanarin-Dorfman syndrome (ichthyosis, deafness, cataracts).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length ABHD5 protein (aa 1-349), which may be suitable for detecting both endogenous and overexpressed ABHD5 in WB.
• For WB, the predicted molecular weight is 39 kDa; verify band specificity by comparing positive and negative control lysates.
• In ELISA, consider using recombinant ABHD5 protein as a standard to generate quantitative data.
• Cross-reactivity with mouse ABHD5 suggests utility in mouse models; however, always validate in the relevant sample matrix to confirm performance.
• Given ABHD5's role in lipid metabolism, consider stimulation with oleic acid or lipolytic agents to observe dynamic changes in protein expression or localization.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized one-stop sourcing bridge for IVD raw materials, CamelBio supports lipid metabolism and dermato-diagnostic research with high-quality antibodies such as ABHD5 Rabbit pAb. We also supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailor-made sourcing for rare targets to streamline your assay development.