ABCC8 Rabbit pAb for WB, ELISA - Q09428

Core Product Specifications and Parameters

Biological Background: ABCC8 Function and Localization

• ABCC8, also known as sulfonylurea receptor 1 (SUR1), is the regulatory subunit of the pancreatic ATP-sensitive potassium channel (KATP).
• It forms a complex with the pore-forming subunit KCNJ11 (Kir6.2) to modulate potassium efflux, thereby linking cellular metabolism to membrane excitability and insulin secretion.
• ABCC8 plays a major role in glucose homeostasis by regulating insulin release from pancreatic beta cells.
• The protein localizes to the cell membrane and contains multiple transmembrane domains and nucleotide-binding motifs essential for its channel-regulatory function.
• Disease-associated variants in ABCC8 are linked to diabetes mellitus and hyperinsulinemic hypoglycemia, underscoring its therapeutic relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (amino acids 700–1000) predicted to lie between transmembrane domains, offering good accessibility for antibody binding in Western blot.
• For ELISA applications, consider using the recombinant immunogen as a positive control to validate assay specificity.
• The predicted molecular weight of ABCC8 is 177 kDa; use an appropriate low-percentage gel and extended transfer conditions for effective Western blot resolution.
• Validate the antibody in your relevant sample system, especially when working with mouse tissues due to confirmed cross-reactivity.

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CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of high-quality IVD raw materials, including validated antibodies for diabetes and metabolic pathways. Our portfolio spans monoclonal and polyclonal antibodies, antigen-grade proteins, and ancillary reagents, all backed by expert technical support. For projects requiring rare or specialized reagents like anti-ABCC8 antibodies, our one-stop sourcing streamlines procurement from concept to clinic.