α-Actin-1 (ACTA1) Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P68133

Core Product Specifications and Parameters

Biological Background: α-Actin-1 (ACTA1) Function and Localization

• α-Actin-1 (ACTA1) is the skeletal muscle-specific alpha-actin isoform, also known as actin, alpha skeletal muscle or Alpha-actin-1, encoded by the ACTA1 gene.
• Actins are a highly conserved protein family involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• ACTA1 is a core component of the cytoskeleton, predominantly localized in the cytoplasm, and forms the thin filaments of muscle fibers essential for contraction.
• It functions as an ATPase (hydrolase activity) and interacts with myosin to generate the force required for muscle contraction.
• Post-translational modifications include acetylation, methylation, and oxidation, which may regulate its function and interactions.
• Mutations in ACTA1 are linked to nemaline myopathy and other congenital myopathies, making it a critical biomarker and research target in muscle disease.
• Keywords associated with ACTA1 encompass 3D-structure, ATP-binding, cytoskeleton, disease variant, hydrolase, and muscle protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–350 of human ACTA1; this C-terminal region may confer specificity for the ACTA1 isoform, but cross-reactivity with other actins should be considered during assay design.
• Recommended applications are WB (expected band at ~42 kDa), IF-P (requires permeabilization), and ELISA; users should validate the antibody in their own sample system.
• Confirmed cross-reactivity with human, mouse, and rat makes it suitable for multi-species studies, but individual target expression should be verified in the relevant tissue or cell type.
• As a rabbit monoclonal antibody, it offers consistent lot-to-lot performance and high specificity; optimal working dilutions must be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio from validated antibody pairs to optimized monoclonal and polyclonal antibodies. This α-Actin-1 (ACTA1) rabbit mAb exemplifies our commitment to supporting skeletal muscle biology and myopathy research, with bulk supplies and ancillary reagents available to streamline your diagnostic development. For rare or custom targets, our technical team bridges the gap from concept to clinic, ensuring you have the critical components needed for every stage of assay development.