647 Rabbit anti-Human BTN3A1/2/3 Monoclonal Antibody for FC - O00481 / P78410 / O00478

Core Product Specifications and Parameters

Biological Background: BTN3A1/2/3 Function and Localization

• BTN3A1 (BTF5, BT3.1, CD277) belongs to the butyrophilin subfamily 3, a group of type I transmembrane proteins containing immunoglobulin-like domains.
• It is crucial for T-cell activation and adaptive immunity, regulating activated T-cell proliferation and cytokine (e.g., IFNG) secretion.
• BTN3A1 mediates T-cell responses against infected and transformed cells, which accumulate phosphorylated metabolites such as isopentenyl pyrophosphate.
• Primarily localized at the cell membrane, consistent with its role in cell-surface signaling and immune cell interaction.
• Detected on immune cells including T cells, NK cells, dendritic cells, and macrophages; highly expressed in heart, pancreas, and lung.
• Features immunoglobulin domains, a transmembrane helix, and a signal peptide; undergoes glycosylation and disulfide bond formation.
• Alternative splicing generates multiple isoforms, potentially modulating tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region of BTN3A1 (aa 470-630), suggesting the antibody targets an epitope within the Ig-like domain; verify staining specificity with appropriate isotype controls and BTN3A-expressing cell lines in flow cytometry.
• Recommended for FC applications; titrate the antibody concentration to achieve optimal signal-to-noise ratios, especially when analyzing PBMCs or tumor-infiltrating lymphocytes.
• Cross-reactivity with BTN3A2/3 isoforms may be advantageous for pan-BTN3 detection; if single-isoform discrimination is needed, consider parallel staining with isoform-specific markers.
• The predicted molecular weight is 58 kDa; for applications beyond FC, validate performance with positive control lysates or cells expressing the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers streamlined access to high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For BTN3A/CD277-related immune function studies, our portfolio includes optimized monoclonal antibodies, recombinant proteins, and customized sourcing solutions. Whether advancing immuno-oncology assays or exploring T-cell biology, we support your project from concept to clinic with reliable, one-stop material supply.